Methylene blue is also known as methylthioninium chloride, is a medication and dye. As a medication, it is mainly used to treat methemoglobinemia. It is specifically used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy.
Methylene blue is widely used as a redox indicator in analytical chemistry. Solutions of this substance are blue when in an oxidizing environment, but will turn colorless if exposed to a reducing agent. An aqueous methylene blue solution containing chloroform can detect anionic surfactants in a water sample. Such a test is known as an MBAS assay.
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